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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated PRDX4 Polyclonal ant (CL488-10703)

    IF Staining of HepG2 using CL488-10703

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PRDX4 antibody (CL488-10703) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated PRDX4 Polyclonal ant (CL488-10703)

    FC experiment of HepG2 using CL488-10703

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PRDX4 (CL488-10703) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PRDX4 Polyclonal antibody

PRDX4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10703

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10703 targets PRDX4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX4 fusion protein Ag1177 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 4
Calculated molecular weight 31 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC007107
Gene Symbol PRDX4
Gene ID (NCBI) 10549
RRIDAB_2934399
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13162
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PRDX4 antibody CL488-10703Download protocol
FC protocol for CL Plus 488 PRDX4 antibody CL488-10703Download protocol
Standard Protocols
Click here to view our Standard Protocols