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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PRDX4 Monoclonal antibody

PRDX4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5B6F1

Cat no : CL594-60286

Synonyms

Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated PRDX4 Monoclonal antibody (CL594-60286)

    IF Staining of HepG2 using CL594-60286

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 PRDX4 antibody (CL594-60286, Clone: 5B6F1 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60286 targets PRDX4 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PRDX4 fusion protein Ag13401 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 4
Calculated molecular weight 31 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC007107
Gene symbol PRDX4
Gene ID (NCBI) 10549
RRIDAB_2883454
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).

Protocols

Product Specific Protocols
IF protocol for CL594 PRDX4 antibody CL594-60286Download protocol
Standard Protocols
Click here to view our Standard Protocols