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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®594-conjugated PRDX6 Monoclonal antibody (CL594-67499)

    IF Staining of A431 using CL594-67499

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 PRDX6 antibody (CL594-67499, Clone: 3C12D3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated PRDX6 Monoclonal antibody (CL594-67499)

    FC experiment of HeLa using CL594-67499

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PRDX6 (CL594-67499, Clone:3C12D3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

CoraLite®594-conjugated PRDX6 Monoclonal antibody

PRDX6 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67499
Clone No.3C12D3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 3C12D3, 1-Cys PRX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67499 targets PRDX6 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PRDX6 fusion protein Ag4727 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 6
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC035857
Gene Symbol PRDX6
Gene ID (NCBI) 9588
RRIDAB_2934769
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30041
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

Protocols

Product Specific Protocols
IF protocol for CL594 PRDX6 antibody CL594-67499Download protocol
Standard Protocols
Click here to view our Standard Protocols