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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PRKACB Polyclonal antibody (12232-1-AP)

    WB analysis of MCF-7 using 12232-1-AP (same clone as 12232-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using PRKACB Polyclonal antibody (12232-1-AP)

    WB analysis of mouse heart using 12232-1-AP (same clone as 12232-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PRKACB Polyclonal antibody (12232-1-AP)

    WB analysis of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PRKACB Polyclonal antibody (12232-1-AP)

    WB analysis of MCF-7 using 12232-1-AP (same clone as 12232-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using PRKACB Polyclonal antibody (12232-1-AP)

    WB analysis of HT-1080 using 12232-1-AP (same clone as 12232-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PRKACB Polyclonal antibody (12232-1-AP)

    WB analysis of A549 using 12232-1-AP (same clone as 12232-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using PRKACB Polyclonal antibody (12232-1-AP)

    WB analysis of PC-3 using 12232-1-AP (same clone as 12232-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PRKACB Polyclonal antibody (12232-1-AP)

    IP experiment of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    IP result of anti-PRKACB (IP:12232-1-AP, 4ug; Detection:12232-1-AP 1:500) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRKACB Polyclonal antibody (12232-1-AP)

    IHC staining of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRKACB Polyclonal antibody (12232-1-AP)

    IHC staining of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human medulloblastoma tissue using PRKACB Polyclonal antibody (12232-1-AP)

    IHC staining of human medulloblastoma using 12232-1-AP (same clone as 12232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12232-1-AP (PRKACB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKACB Polyclonal antibody (12232-1-AP)

    IF Staining of HeLa using 12232-1-AP (same clone as 12232-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKACB antibody (12232-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

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PRKACB Polyclonal antibody, PBS Only

PRKACB Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12232-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12232-1-PBS targets PRKACB in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKACB fusion protein Ag2870 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, catalytic, beta
Calculated molecular weight 41 kDa, 46 kDa
Observed molecular weight 36-55 kDa
GenBank accession numberBC016285
Gene Symbol PRKACB
Gene ID (NCBI) 5567
RRIDAB_2170177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22694
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.