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KD/KO Validated

PKC Iota Polyclonal antibody

Name: PKC Iota Polyclonal antibody
Brand: Proteintech
SKU: 13883-1-AP

PKC Iota Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13883-1-AP

Synonyms

PRKCI, nPKC-iota, EC:2.7.11.13, DXS1179E, Atypical protein kinase C-lambda/iota

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 13883-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13883-1-AP (PKC Iota antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13883-1-AP
WB result of PKC Iota antibody (13883-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected MCF-7 cells.
IP experiment of HeLa using 13883-1-AP
IP result of anti-PKC Iota (IP:13883-1-AP, 4ug; Detection:13883-1-AP 1:2000) with HeLa cells lysate 1720 ug.
IHC staining of human breast cancer using 13883-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13883-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13883-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13883-1-AP (PKC iota antibody at dilution of 1:200 (under 40x lens).
IHC staining of human lung cancer using 13883-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13883-1-AP (PKC iota antibody at dilution of 1:200 (under 10x lens).
IF Staining of HeLa using 13883-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Iota antibody (13883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 13883-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PKC Iota (13883-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 13883-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 6 publications below
IHC	See 4 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

13883-1-AP targets PKC Iota in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PKC Iota fusion protein Ag4990 相同性解析による交差性が予測される生物種
Full Name	protein kinase C, iota
Calculated molecular weight	68 kDa
Observed molecular weight	67 kDa
GenBank accession number	BC022016
Gene symbol	PKC Iota
Gene ID (NCBI)	5584
RRID	AB_2171777
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Iota antibody 13883-1-AP	Download protocol
IHC protocol for PKC Iota antibody 13883-1-AP	Download protocol
IF protocol for PKC Iota antibody 13883-1-AP	Download protocol
IP protocol for PKC Iota antibody 13883-1-AP	Download protocol
FC protocol for PKC Iota antibody 13883-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Mol Cancer circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway. Authors - Wei Gao KD Validated View Article
chicken	IHC,IF	Development Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch. Authors - Jianqiong Lin KD Validated View Article
human,mouse	WB,IHC,IF,CoIP	Mol Med Paired protein kinases PRKCI-RIPK2 promote pancreatic cancer growth and metastasis via enhancing NF-κB/JNK/ERK phosphorylation Authors - Juying Jiao View Article
human	WB	Int J Biol Sci Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation. Authors - Fangfei Li View Article
human	WB,IHC	Onco Targets Ther Protein kinase C-iota-mediated glycolysis promotes non-small-cell lung cancer progression. Authors - Liu Liu KD Validated View Article
human	WB,IHC,IP	Antioxidants (Basel) PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4 Authors - Hao Tao KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKC Iota Polyclonal antibody

PKC Iota Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 13883-1-AP

WB analysis of MCF-7 using 13883-1-AP

IP experiment of HeLa using 13883-1-AP

IHC staining of human breast cancer using 13883-1-AP

IHC staining of human breast cancer using 13883-1-AP

IHC staining of human lung cancer using 13883-1-AP

IHC staining of human lung cancer using 13883-1-AP

IF Staining of HeLa using 13883-1-AP

FC experiment of HeLa using 13883-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway.

Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Paired protein kinases PRKCI-RIPK2 promote pancreatic cancer growth and metastasis via enhancing NF-κB/JNK/ERK phosphorylation

Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation.

Protein kinase C-iota-mediated glycolysis promotes non-small-cell lung cancer progression.

PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4