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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PKC Iota Polyclonal antibody

PKC Iota Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13883

Synonyms

PRKCI, nPKC-iota, EC:2.7.11.13, DXS1179E, Atypical protein kinase C-lambda/iota

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PKC Iota Polyclonal (CL488-13883)

    IF Staining of HeLa using CL488-13883

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PKC Iota antibody (CL488-13883) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PKC Iota Polyclonal (CL488-13883)

    FC experiment of HeLa using CL488-13883

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PKC Iota (CL488-13883) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13883 targets PKC Iota in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Iota fusion protein Ag4990 相同性解析による交差性が予測される生物種
Full Name protein kinase C, iota
Calculated molecular weight 68 kDa
GenBank accession numberBC022016
Gene symbol PKC Iota
Gene ID (NCBI) 5584
RRIDAB_2919080
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PKC Iota antibody CL488-13883Download protocol
FC protocol for CL Plus 488 PKC Iota antibody CL488-13883Download protocol
Standard Protocols
Click here to view our Standard Protocols