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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKD1 Recombinant antibody, PBS Only

PRKD1 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230355D12

Cat no : 83174-5-PBS

Synonyms

Serine/threonine protein kinase D1, PRKCM, PKD1, PKD, 230355D12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKD1 Recombinant antibody (83174-5-RR)

    IF Staining of HeLa using 83174-5-RR (same clone as 83174-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRKD1 antibody (83174-5-RR, Clone: 230355D12 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83174-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PRKD1 Recombinant antibody (83174-5-RR)

    FC experiment of HeLa using 83174-5-RR (same clone as 83174-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Prkd1 Recombinant Antibody (83174-5-RR, Clone:230355D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83174-5-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83174-5-PBS targets PRKD1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase D1
Calculated molecular weight 102 kDa
GenBank accession numberNM_002742
Gene symbol PRKD1
Gene ID (NCBI) 5587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.