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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKD1 Recombinant antibody

PRKD1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230355D12

Cat no : 83174-5-RR

Synonyms

Serine/threonine protein kinase D1, PRKCM, PKD1, PKD, 230355D12

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKD1 Recombinant antibody (83174-5-RR)

    IF Staining of HeLa using 83174-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRKD1 antibody (83174-5-RR, Clone: 230355D12 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using PRKD1 Recombinant antibody (83174-5-RR)

    FC experiment of HeLa using 83174-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Prkd1 Recombinant Antibody (83174-5-RR, Clone:230355D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83174-5-RR targets PRKD1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase D1
Calculated molecular weight 102 kDa
GenBank accession numberNM_002742
Gene symbol PRKD1
Gene ID (NCBI) 5587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PRKD1 antibody 83174-5-RRDownload protocol
FC protocol for PRKD1 antibody 83174-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols