Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, A431 cells, T-47D cells, 4T1 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
67292-1-Ig targets PRLR in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PRLR fusion protein Ag28778 相同性解析による交差性が予測される生物種 |
| Full Name | prolactin receptor |
| Calculated molecular weight | 622 aa, 70 kDa |
| Observed molecular weight | 45 kDa, 70 kDa, 85-85 kDa |
| GenBank accession number | BC059392 |
| Gene Symbol | PRLR |
| Gene ID (NCBI) | 5618 |
| RRID | AB_2882557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P16471 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRLR belongs to the cytokine type I subfamily, which also includes receptors for leptin, leukemia inhibiting factor, erythropoietin and so on. PRLR has long and short forms at approximately 70 and 45 kDa, respectively, and the mature, glycosylated form of PRLR migrates at 85-95 kDa in human decidua and placenta (PMID: 18258683).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRLR antibody 67292-1-Ig | Download protocol |
| IHC protocol for PRLR antibody 67292-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Genet What Mediates Fibrosis in the Tumor Microenvironment of Clear Renal Cell Carcinoma. | ||
Front Vet Sci Hybrid Broussonetia papyrifera Fermented Feed Can Play a Role Through Flavonoid Extracts to Increase Milk Production and Milk Fatty Acid Synthesis in Dairy Goats. |