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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRLR Monoclonal antibody, PBS Only

PRLR Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E10G5

Cat no : 67292-1-PBS

Synonyms

hPRLrI, PRL R, PRLR, prolactin receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRLR Monoclonal antibody (67292-1-Ig)

    WB analysis using 67292-1-Ig (same clone as 67292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PRLR Monoclonal antibody (67292-1-Ig)

    WB analysis of A431 using 67292-1-Ig (same clone as 67292-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using PRLR Monoclonal antibody (67292-1-Ig)

    WB analysis of T-47D using 67292-1-Ig (same clone as 67292-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRLR Monoclonal antibody (67292-1-Ig)

    IHC staining of human breast cancer using 67292-1-Ig (same clone as 67292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRLR Monoclonal antibody (67292-1-Ig)

    IHC staining of human breast cancer using 67292-1-Ig (same clone as 67292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67292-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67292-1-PBS targets PRLR in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRLR fusion protein Ag28778 相同性解析による交差性が予測される生物種
Full Name prolactin receptor
Calculated molecular weight 622 aa, 70 kDa
Observed molecular weight 45 kDa, 70 kDa, 85-85 kDa
GenBank accession numberBC059392
Gene symbol PRLR
Gene ID (NCBI) 5618
RRIDAB_2882557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRLR belongs to the cytokine type I subfamily, which also includes receptors for leptin, leukemia inhibiting factor, erythropoietin and so on. PRLR has long and short forms at approximately 70 and 45 kDa, respectively, and the mature, glycosylated form of PRLR migrates at 85-95 kDa in human decidua and placenta (PMID: 18258683).