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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRM2 Polyclonal antibody, PBS Only

PRM2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14500-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using PRM2 Polyclonal antibody (14500-1-AP)

    WB analysis of human testis using 14500-1-AP (same clone as 14500-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 14500-1-AP (PRM2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRM2 Polyclonal antibody (14500-1-AP)

    IHC staining of human testis using 14500-1-AP (same clone as 14500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRM2 Polyclonal antibody (14500-1-AP)

    IHC staining of human testis using 14500-1-AP (same clone as 14500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14500-1-AP (PRM2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14500-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14500-1-PBS targets PRM2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRM2 fusion protein Ag5952 相同性解析による交差性が予測される生物種
Full Name protamine 2
Calculated molecular weight 13 kDa
Observed molecular weight 10-25 kDa
GenBank accession numberBC066338
Gene symbol PRM2
Gene ID (NCBI) 5620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRM2 (Protamine-2) is the main DNA-binding protein in the sperm nucleus, consisting of a C-terminal mature PRM2 (mPRM2) domain and an N-terminal lytic PRM2 (cPRM2) domain, which is lyzed sequentially after binding to DNA. PRM2 plays an important role in spermatogenesis and development. PRM2 is specifically expressed in sperm and localized in the sperm nucleus.