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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRODH2 Polyclonal antibody (31047-1-AP)

    WB analysis using 31047-1-AP (same clone as 31047-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31047-1-AP (PRODH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31047-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRODH2 Polyclonal antibody (31047-1-AP)

    WB analysis using 31047-1-AP (same clone as 31047-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31047-1-AP (PRODH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31047-1-PBS in a different storage buffer formulation.

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PRODH2 Polyclonal antibody, PBS Only

PRODH2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 31047-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31047-1-PBS targets PRODH2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRODH2 fusion protein Ag33970 相同性解析による交差性が予測される生物種
Full Name proline dehydrogenase (oxidase) 2
Observed molecular weight 50 kDa
GenBank accession numberBC166629
Gene Symbol PRODH2
Gene ID (NCBI) 58510
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UF12
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRODH2 is a dehydrogenase that converts trans-4-L-hydroxyproline to delta-1-pyrroline-3-hydroxy-5-carboxylate (Hyp) using ubiquinone-10 as the terminal electron acceptor. It can also use proline as a substrate but with a very much lower efficiency (PMID: 25697095).