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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PROS1 Recombinant antibody, PBS Only (Capture)

PROS1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241241E5

Cat no : 84107-2-PBS

Synonyms

Vitamin K-dependent protein S, PROS, 241241E5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PROS1 Recombinant antibody (84107-2-RR)

    WB analysis using 84107-2-RR (same clone as 84107-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84107-2-RR (PROS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01044-1

    Sandwich ELISA standard curve of MP01044-1

    Sandwich ELISA standard curve of MP01044-1, Human PROS1 Recombinant Matched Antibody Pair - PBS only. 84107-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10539. 84107-1-PBS was HRP conjugated as the detection antibody. Range: 0.469-30 ng/mL

  • Sample test of MP01044-1

    Sample test of MP01044-1

    The mean PROS1 concentration was determined to be 276.09 ng/mL in HuH-7 cell extract based on a 1.4 mg/mL extract load.

  • Flow cytometry (FC) experiment of A549 cells using PROS1 Recombinant antibody (84107-2-RR)

    FC experiment of A549 using 84107-2-RR (same clone as 84107-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PROS1 Recombinant antibody (84107-2-RR, Clone:241241E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84107-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01044-1

    Cytometric bead array standard curve of MP01044-1

    Cytometric bead array standard curve of MP01044-1, PROS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84107-2-PBS. Detection antibody: 84107-1-PBS. Standard: Ag10539. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84107-2-RR

    Affinity and Kinetic Characterization of 84107-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84107-2-RR against Human PROS1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84107-2-PBS targets PROS1 as part of a matched antibody pair:

MP01044-1: 84107-2-PBS capture and 84107-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PROS1 fusion protein Ag10539 相同性解析による交差性が予測される生物種
Full Name protein S (alpha)
Calculated molecular weight 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC015801
Gene symbol PROS1
Gene ID (NCBI) 5627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.