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Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using PROS1 Recombinant antibody (84107-2-RR)

    WB analysis of DU 145 using 84107-2-RR

    WB result of PROS1 antibody (84107-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PROS1 transfected DU 145 cells.

  • Western Blot (WB) analysis of various lysates using PROS1 Recombinant antibody (84107-2-RR)

    WB analysis using 84107-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84107-2-RR (PROS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PROS1 Recombinant antibody (84107-2-RR)

    IHC staining of mouse brain using 84107-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84107-2-RR (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PROS1 Recombinant antibody (84107-2-RR)

    IHC staining of mouse brain using 84107-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84107-2-RR (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PROS1 Recombinant antibody (84107-2-RR)

    IF Staining of HepG2 using 84107-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PROS1 antibody (84107-2-RR, Clone: 241241E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PROS1 Recombinant antibody (84107-2-RR)

    IF Staining of HepG2 using 84107-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (84107-2-RR, Clone: 241241E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using PROS1 Recombinant antibody (84107-2-RR)

    FC experiment of A549 using 84107-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PROS1 Recombinant antibody (84107-2-RR, Clone:241241E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84107-2-RR

    Affinity and Kinetic Characterization of 84107-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84107-2-RR against Human PROS1 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

PROS1 Recombinant antibody

PROS1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84107-2-RR
Clone No.241241E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Vitamin K-dependent protein S, PROS, 241241E5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, PC-3 cells, HuH-7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84107-2-RR targets PROS1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PROS1 fusion protein Ag10539 相同性解析による交差性が予測される生物種
Full Name protein S (alpha)
Calculated molecular weight 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC015801
Gene Symbol PROS1
Gene ID (NCBI) 5627
RRIDAB_3671673
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07225
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

Protocols

Product Specific Protocols
WB protocol for PROS1 antibody 84107-2-RRDownload protocol
IHC protocol for PROS1 antibody 84107-2-RRDownload protocol
IF protocol for PROS1 antibody 84107-2-RRDownload protocol
FC protocol for PROS1 antibody 84107-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols