CoraLite® Plus 488-conjugated PROX1 Monoclonal antibody
PROX1 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D4B1
Cat no : CL488-67438
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HuH-7 cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67438 targets PROX1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | PROX1 fusion protein Ag9681 相同性解析による交差性が予測される生物種 |
Full Name | prospero homeobox 1 |
Calculated molecular weight | 737 aa, 83 kDa |
Observed molecular weight | 90 kDa |
GenBank accession number | BC024201 |
Gene symbol | PROX1 |
Gene ID (NCBI) | 5629 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 PROX1 antibody CL488-67438 | Download protocol |
FC protocol for CL Plus 488 PROX1 antibody CL488-67438 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |