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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PRX5 Monoclonal antibody (67599-1-Ig)

    WB analysis of HepG2 using 67599-1-Ig (same clone as 67599-1-PBS)

    WB result of PRX5 antibody (67599-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRX5 transfected HepG2 cells. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRX5 Monoclonal antibody (67599-1-Ig)

    WB analysis using 67599-1-Ig (same clone as 67599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRX5 Monoclonal antibody (67599-1-Ig)

    WB analysis using 67599-1-Ig (same clone as 67599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRX5 Monoclonal antibody (67599-1-Ig)

    IHC staining of human liver cancer using 67599-1-Ig (same clone as 67599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRX5 Monoclonal antibody (67599-1-Ig)

    IHC staining of human liver cancer using 67599-1-Ig (same clone as 67599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRX5 Monoclonal antibody (67599-1-Ig)

    IF Staining of HeLa using 67599-1-Ig (same clone as 67599-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRX5 antibody (67599-1-Ig, Clone: 1B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PRX5 Monoclonal antibody, PBS Only

PRX5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67599-1-PBS
Clone No.1B8C5

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ACR1, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, AOPP, B166, Liver tissue 2D page spot 71B, peroxiredoxin 5, Peroxiredoxin 5, mitochondrial, Peroxiredoxin V, Peroxisomal antioxidant enzyme, PLP, PMP20, PRDX5, PRDX6, Prx V, Prx5, PRXV, SBBI10, Thioredoxin peroxidase PMP20, Thioredoxin reductase, TPx type VI

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67599-1-PBS targets PRX5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRX5 fusion protein Ag12262 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 5
Calculated molecular weight 214 aa, 22 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC110983
Gene Symbol PRX5
Gene ID (NCBI) 25824
RRIDAB_2882805
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30044
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)