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PRX5 Monoclonal antibody

PRX5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8C5

Cat no : 67599-1-Ig

Synonyms

ACR1, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, AOPP, B166, Liver tissue 2D page spot 71B, peroxiredoxin 5, Peroxiredoxin 5, mitochondrial, Peroxiredoxin V, Peroxisomal antioxidant enzyme, PLP, PMP20, PRDX5, PRDX6, Prx V, Prx5, PRXV, SBBI10, Thioredoxin peroxidase PMP20, Thioredoxin reductase, TPx type VI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRX5 Monoclonal antibody (67599-1-Ig)

    WB analysis using 67599-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRX5 Monoclonal antibody (67599-1-Ig)

    WB analysis using 67599-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PRX5 Monoclonal antibody (67599-1-Ig)

    WB analysis of HepG2 using 67599-1-Ig

    WB result of PRX5 antibody (67599-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRX5 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRX5 Monoclonal antibody (67599-1-Ig)

    IHC staining of human liver cancer using 67599-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRX5 Monoclonal antibody (67599-1-Ig)

    IHC staining of human liver cancer using 67599-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRX5 Monoclonal antibody (67599-1-Ig)

    IF Staining of HeLa using 67599-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRX5 antibody (67599-1-Ig, Clone: 1B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHCT 116 cells, LNCaP cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10400
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67599-1-Ig targets PRX5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRX5 fusion protein Ag12262 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 5
Calculated molecular weight 214 aa, 22 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC110983
Gene symbol PRX5
Gene ID (NCBI) 25824
RRIDAB_2882805
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)

Protocols

Product Specific Protocols
WB protocol for PRX5 antibody 67599-1-IgDownload protocol
IHC protocol for PRX5 antibody 67599-1-IgDownload protocol
IF protocol for PRX5 antibody 67599-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang
View Article