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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Presenilin-1-Specific Recombinant antibody, PBS Only

Presenilin-1-Specific Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240615B4

Cat no : 83618-3-PBS

Synonyms

PSEN1-Specific, PSEN1, Presenilin-1 CTF12, Presenilin-1 CTF subunit, Presenilin-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    WB analysis using 83618-3-RR (same clone as 83618-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83618-3-RR (PSEN1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83618-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    IHC staining of mouse brain using 83618-3-RR (same clone as 83618-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-3-RR (Presenilin-1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83618-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    IHC staining of mouse brain using 83618-3-RR (same clone as 83618-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-3-RR (Presenilin-1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83618-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    IF Staining of rat brain using 83618-3-RR (same clone as 83618-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Presenilin-1-Specific antibody (83618-3-RR, Clone: 240615B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83618-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    IF Staining of rat brain using 83618-3-RR (same clone as 83618-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Presenilin-1-Specific antibody (83618-3-RR, Clone: 240615B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83618-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83618-3-PBS targets Presenilin-1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name presenilin 1
Calculated molecular weight 53 kDa
Observed molecular weight53 kda
GenBank accession numberNM_000021
Gene symbol PSEN1
Gene ID (NCBI) 5663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.