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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Presenilin-1-Specific Recombinant antibody

Presenilin-1-Specific Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240615B4

Cat no : 83618-3-RR

Synonyms

PSEN1, Presenilin-1 CTF12, Presenilin-1 CTF subunit, Presenilin-1, EC:3.4.23.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    WB analysis using 83618-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83618-3-RR (PSEN1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    IHC staining of mouse brain using 83618-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-3-RR (Presenilin-1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    IHC staining of mouse brain using 83618-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-3-RR (Presenilin-1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    IF Staining of rat brain using 83618-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Presenilin-1-Specific antibody (83618-3-RR, Clone: 240615B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Presenilin-1-Specific Recombinant antibody (83618-3-RR)

    IF Staining of rat brain using 83618-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Presenilin-1-Specific antibody (83618-3-RR, Clone: 240615B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Caco-2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83618-3-RR targets Presenilin-1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name presenilin 1
Calculated molecular weight 53 kDa
Observed molecular weight53 kda
GenBank accession numberNM_000021
Gene symbol PSEN1
Gene ID (NCBI) 5663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.

Protocols

Product Specific Protocols
WB protocol for Presenilin-1-Specific antibody 83618-3-RRDownload protocol
IHC protocol for Presenilin-1-Specific antibody 83618-3-RRDownload protocol
IF protocol for Presenilin-1-Specific antibody 83618-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols