• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMA/GCPII Monoclonal antibody, PBS Only

PSMA/GCPII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G4E12

Cat no : 66678-1-PBS

Synonyms

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of LNCaP cells using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    WB analysis of LNCaP using 66678-1-Ig (same clone as 66678-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    WB analysis of rat kidney using 66678-1-Ig (same clone as 66678-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IF Staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IF Staining of PC-3 using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:90 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IF Staining of PC-3 using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66678-1-PBS targets PSMA/GCPII in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMA/GCPII fusion protein Ag16594 相同性解析による交差性が予測される生物種
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC025672
Gene symbol PSMA
Gene ID (NCBI) 2346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.