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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3G4E12

Cat no : 66678-1-Ig

Synonyms

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    WB analysis of LNCaP using 66678-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    WB analysis of rat kidney using 66678-1-Ig

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IHC staining of human prostate cancer using 66678-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IHC staining of human prostate cancer using 66678-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IF Staining of human prostate cancer using 66678-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IF Staining of PC-3 using 66678-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:90 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PSMA/GCPII Monoclonal antibody (66678-1-Ig)

    IF Staining of PC-3 using 66678-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, rat kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue, PC-3 cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66678-1-Ig targets PSMA/GCPII in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMA/GCPII fusion protein Ag16594 相同性解析による交差性が予測される生物種
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC025672
Gene symbol PSMA
Gene ID (NCBI) 2346
RRIDAB_2882032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
IHC protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
IF protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Cancer cell's neuroendocrine feature can be acquired through cell-cell fusion during cancer-neural stem cell interaction.

Authors - Liyuan Yin
View Article