CoraLite® Plus 488-conjugated PSMA/GCPII Monoclonal antibody
PSMA/GCPII Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, rat
Applications
IF/ICC, IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3G4E12
Cat no : CL488-66678
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human prostate cancer tissue, PC-3 cells |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66678 targets PSMA/GCPII in IF/ICC, IF-P applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PSMA/GCPII fusion protein Ag16594 相同性解析による交差性が予測される生物種 |
| Full Name | folate hydrolase (prostate-specific membrane antigen) 1 |
| Calculated molecular weight | 719 aa, 81 kDa |
| Observed molecular weight | 100-120 kDa |
| GenBank accession number | BC025672 |
| Gene symbol | PSMA |
| Gene ID (NCBI) | 2346 |
| RRID | AB_2919356 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PSMA/GCPII antibody CL488-66678 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
