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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using CoraLite®594-conjugated PSMA/GCPII Monoclonal anti (CL594-66678)

    IF Staining of human prostate cancer using CL594-66678

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®594 PSMA/GCPII antibody (CL594-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite®594-conjugated PSMA/GCPII Monoclonal anti (CL594-66678)

    IF Staining of PC-3 using CL594-66678

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite®594 PSMA/GCPII antibody (CL594-66678, Clone: 3G4E12 ) at dilution of 1:200.

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CoraLite®594-conjugated PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL594-66678
Clone No.3G4E12

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, IF-P

Synonyms

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inPC-3 cells, human prostate cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66678 targets PSMA/GCPII in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMA/GCPII fusion protein Ag16594 相同性解析による交差性が予測される生物種
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC025672
Gene Symbol PSMA
Gene ID (NCBI) 2346
RRIDAB_2920020
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ04609
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 PSMA/GCPII antibody CL594-66678Download protocol
Standard Protocols
Click here to view our Standard Protocols