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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMA6 Monoclonal antibody, PBS Only

PSMA6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D4B2

Cat no : 67695-1-PBS

Synonyms

PROS-27, PROS27, PROS 27, p27K, IOTA

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PSMA6 Monoclonal antibody (67695-1-Ig)

    WB analysis using 67695-1-Ig (same clone as 67695-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67695-1-Ig (PSMA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSMA6 Monoclonal antibody (67695-1-Ig)

    IHC staining of human lung cancer using 67695-1-Ig (same clone as 67695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67695-1-Ig (PSMA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSMA6 Monoclonal antibody (67695-1-Ig)

    IHC staining of human lung cancer using 67695-1-Ig (same clone as 67695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67695-1-Ig (PSMA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using PSMA6 Monoclonal antibody (67695-1-Ig)

    IF Staining of human lung cancer using 67695-1-Ig (same clone as 67695-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMA6 antibody (67695-1-Ig, Clone: 1D4B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA6 Monoclonal antibody (67695-1-Ig)

    IF Staining of HeLa using 67695-1-Ig (same clone as 67695-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA6 antibody (67695-1-Ig, Clone: 1D4B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PSMA6 Monoclonal antibody (67695-1-Ig)

    FC experiment of HepG2 using 67695-1-Ig (same clone as 67695-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PSMA6 (67695-1-Ig, Clone:1D4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67695-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67695-1-PBS targets PSMA6 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PSMA6 fusion protein Ag21761 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 6
Calculated molecular weight 246 aa, 27 kDa
Observed molecular weight30 kDa
GenBank accession numberBC023659
Gene symbol PSMA6
Gene ID (NCBI) 5687
RRIDAB_2882887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.