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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMA6 Monoclonal antibody

PSMA6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D4B2

Cat no : 67695-1-Ig

Synonyms

PROS-27, PROS27, PROS 27, p27K, IOTA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA6 Monoclonal antibody (67695-1-Ig)

    WB analysis using 67695-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67695-1-Ig (PSMA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSMA6 Monoclonal antibody (67695-1-Ig)

    IHC staining of human lung cancer using 67695-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67695-1-Ig (PSMA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSMA6 Monoclonal antibody (67695-1-Ig)

    IHC staining of human lung cancer using 67695-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67695-1-Ig (PSMA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using PSMA6 Monoclonal antibody (67695-1-Ig)

    IF Staining of human lung cancer using 67695-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMA6 antibody (67695-1-Ig, Clone: 1D4B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA6 Monoclonal antibody (67695-1-Ig)

    IF Staining of HeLa using 67695-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA6 antibody (67695-1-Ig, Clone: 1D4B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using PSMA6 Monoclonal antibody (67695-1-Ig)

    FC experiment of HepG2 using 67695-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PSMA6 (67695-1-Ig, Clone:1D4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67695-1-Ig targets PSMA6 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PSMA6 fusion protein Ag21761 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 6
Calculated molecular weight 246 aa, 27 kDa
Observed molecular weight30 kDa
GenBank accession numberBC023659
Gene symbol PSMA6
Gene ID (NCBI) 5687
RRIDAB_2882887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.

Protocols

Product Specific Protocols
WB protocol for PSMA6 antibody 67695-1-IgDownload protocol
IHC protocol for PSMA6 antibody 67695-1-IgDownload protocol
IF protocol for PSMA6 antibody 67695-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols