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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite® Plus 488-conjugated PSMA6 Monoclonal ant (CL488-67695)

    IF Staining of human lung cancer using CL488-67695

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 PSMA6 antibody (CL488-67695, Clone: 1D4B2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated PSMA6 Monoclonal ant (CL488-67695)

    IF Staining of U2OS using CL488-67695

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 PSMA6 antibody (CL488-67695, Clone: 1D4B2 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated PSMA6 Monoclonal antibody

PSMA6 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-67695
Clone No.1D4B2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Synonyms

PROS-27, PROS27, PROS 27, p27K, IOTA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67695 targets PSMA6 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PSMA6 fusion protein Ag21761 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 6
Calculated molecular weight 246 aa, 27 kDa
Observed molecular weight30 kDa
GenBank accession numberBC023659
Gene Symbol PSMA6
Gene ID (NCBI) 5687
RRIDAB_2919523
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60900
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PSMA6 antibody CL488-67695Download protocol
Standard Protocols
Click here to view our Standard Protocols