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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB5 Monoclonal antibody (67959-1-Ig)

    WB analysis using 67959-1-Ig (same clone as 67959-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67959-1-Ig (PSMB5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PSMB5 Monoclonal antibody (67959-1-Ig)

    WB analysis of HeLa using 67959-1-Ig (same clone as 67959-1-PBS)

    WB result of PSMB5 antibody (67959-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells. This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PSMB5 Monoclonal antibody (67959-1-Ig)

    IHC staining of human breast cancer using 67959-1-Ig (same clone as 67959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PSMB5 Monoclonal antibody (67959-1-Ig)

    IHC staining of human breast cancer using 67959-1-Ig (same clone as 67959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMB5 Monoclonal antibody (67959-1-Ig)

    IF Staining of HeLa using 67959-1-Ig (same clone as 67959-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB5 antibody (67959-1-Ig, Clone: 2E9H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PSMB5 Monoclonal antibody (67959-1-Ig)

    FC experiment of HepG2 using 67959-1-Ig (same clone as 67959-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMB5 Monoclonal antibody (67959-1-Ig, Clone:2E9H1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67959-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PSMB5 Monoclonal antibody, PBS Only

PSMB5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67959-1-PBS
Clone No.2E9H1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

X, Proteasome subunit beta type-5, MB1, LMPX, EC:3.4.25.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67959-1-PBS targets PSMB5 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PSMB5 fusion protein Ag5828 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 5
Calculated molecular weight 263 aa, 28 kDa
Observed molecular weight22 kDa
GenBank accession numberBC057840
Gene Symbol PSMB5
Gene ID (NCBI) 5693
RRIDAB_2918710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28074
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.