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PSMB5 Monoclonal antibody

PSMB5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E9H1

Cat no : 67959-1-Ig

Synonyms

LMPX, Macropain epsilon chain, MB1, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit beta type 5, Proteasome subunit MB1, Proteasome subunit X, PSMB5, X

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB5 Monoclonal antibody (67959-1-Ig)

    WB analysis using 67959-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67959-1-Ig (PSMB5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HeLa cells using PSMB5 Monoclonal antibody (67959-1-Ig)

    WB analysis of HeLa using 67959-1-Ig

    WB result of PSMB5 antibody (67959-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PSMB5 Monoclonal antibody (67959-1-Ig)

    IHC staining of human breast cancer using 67959-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PSMB5 Monoclonal antibody (67959-1-Ig)

    IHC staining of human breast cancer using 67959-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67959-1-Ig (PSMB5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMB5 Monoclonal antibody (67959-1-Ig)

    IF Staining of HeLa using 67959-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB5 antibody (67959-1-Ig, Clone: 2E9H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, U2OS cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67959-1-Ig targets PSMB5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PSMB5 fusion protein Ag5828 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 5
Calculated molecular weight 263 aa, 28 kDa
Observed molecular weight22 kDa
GenBank accession numberBC057840
Gene symbol PSMB5
Gene ID (NCBI) 5693
RRIDAB_2918710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PSMB5 antibody 67959-1-IgDownload protocol
IHC protocol for PSMB5 antibody 67959-1-IgDownload protocol
IF protocol for PSMB5 antibody 67959-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols