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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PSMB5 Monoclonal antibody

PSMB5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E9H1

Cat no : CL488-67959

Synonyms

X, Proteasome subunit beta type-5, MB1, LMPX, EC:3.4.25.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PSMB5 Monoclonal ant (CL488-67959)

    IF Staining of HeLa using CL488-67959

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PSMB5 antibody (CL488-67959, Clone: 2E9H1 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PSMB5 Monoclonal ant (CL488-67959)

    FC experiment of HeLa using CL488-67959

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated PSMB5 Monoclonal antibody (CL488-67959, Clone:2E9H1)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67959 targets PSMB5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PSMB5 fusion protein Ag5828 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 5
Calculated molecular weight 263 aa, 28 kDa
Observed molecular weight22 kDa
GenBank accession numberBC057840
Gene symbol PSMB5
Gene ID (NCBI) 5693
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PSMB5 antibody CL488-67959Download protocol
Standard Protocols
Click here to view our Standard Protocols