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PSME1 Monoclonal antibody

PSME1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F7E2

Cat no : 67928-1-Ig

Synonyms

IFI5111, IGUP I 5111, PA28A, PA28alpha, PSME1, REG alpha, REGalpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSME1 Monoclonal antibody (67928-1-Ig)

    WB analysis using 67928-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67928-1-Ig (PSME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of HeLa cells using PSME1 Monoclonal antibody (67928-1-Ig)

    WB analysis of HeLa using 67928-1-Ig

    WB result of PSME1 antibody (67928-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSME1 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PSME1 Monoclonal antibody (67928-1-Ig)

    IF Staining of MCF-7 using 67928-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME1 antibody (67928-1-Ig, Clone: 1F7E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inMDA-MB-231 cells, Hela cells, HEK-293 cells, Jurkat cells, k-562 cells, HSC-T6 cells, C6 cells, NIH/3T3 cells and 4T1 cells.
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67928-1-Ig targets PSME1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSME1 fusion protein Ag30549 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Calculated molecular weight 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC007503
Gene symbol PSME1
Gene ID (NCBI) 5720
RRIDAB_2918680
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Protocols

Product Specific Protocols
WB protocol for PSME1 antibody 67928-1-IgDownload protocol
IF protocol for PSME1 antibody 67928-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols