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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSME1 Monoclonal antibody (67928-1-Ig)

    WB analysis using 67928-1-Ig (same clone as 67928-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67928-1-Ig (PSME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PSME1 Monoclonal antibody (67928-1-Ig)

    WB analysis of HeLa using 67928-1-Ig (same clone as 67928-1-PBS)

    WB result of PSME1 antibody (67928-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSME1 transfected HeLa cells. This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PSME1 Monoclonal antibody (67928-1-Ig)

    IF Staining of MCF-7 using 67928-1-Ig (same clone as 67928-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME1 antibody (67928-1-Ig, Clone: 1F7E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67928-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PSME1 Monoclonal antibody, PBS Only

PSME1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67928-1-PBS
Clone No.1F7E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

REG-alpha, PA28A, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67928-1-PBS targets PSME1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSME1 fusion protein Ag30549 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Calculated molecular weight 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC007503
Gene Symbol PSME1
Gene ID (NCBI) 5720
RRIDAB_2918680
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ06323
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.