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PSPH Monoclonal antibody

PSPH Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B12G3

Cat no : 68500-1-Ig

Synonyms

L 3 phosphoserine phosphatase, phosphoserine phosphatase, PSP, PSPase, PSPH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSPH Monoclonal antibody (68500-1-Ig)

    WB analysis using 68500-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68500-1-Ig (PSPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using PSPH Monoclonal antibody (68500-1-Ig)

    WB analysis of A375 using 68500-1-Ig

    WB result of PSPH antibody (68500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPH transfected A375 cells.

  • Immunoprecipitation (IP) experiment of HL-60 cells using PSPH Monoclonal antibody (68500-1-Ig)

    IP experiment of HL-60 using 68500-1-Ig

    IP result of anti-PSPH (IP:68500-1-Ig, 4ug; Detection:68500-1-Ig 1:5000) with HL-60 cells lysate 2440 ug.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PSPH Monoclonal antibody (68500-1-Ig)

    IHC staining of mouse spleen using 68500-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68500-1-Ig (PSPH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSPH Monoclonal antibody (68500-1-Ig)

    IF Staining of HepG2 using 68500-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (68500-1-Ig, Clone: 1B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, A375 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHL-60 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68500-1-Ig targets PSPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSPH fusion protein Ag6070 相同性解析による交差性が予測される生物種
Full Name phosphoserine phosphatase
Calculated molecular weight 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC063614
Gene symbol PSPH
Gene ID (NCBI) 5723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).

Protocols

Product Specific Protocols
WB protocol for PSPH antibody 68500-1-IgDownload protocol
IHC protocol for PSPH antibody 68500-1-IgDownload protocol
IF protocol for PSPH antibody 68500-1-IgDownload protocol
IP protocol for PSPH antibody 68500-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols