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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSPH Monoclonal antibody (68500-1-Ig)

    WB analysis using 68500-1-Ig (same clone as 68500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68500-1-Ig (PSPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using PSPH Monoclonal antibody (68500-1-Ig)

    WB analysis of A375 using 68500-1-Ig (same clone as 68500-1-PBS)

    WB result of PSPH antibody (68500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPH transfected A375 cells. This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using PSPH Monoclonal antibody (68500-1-Ig)

    IP experiment of HL-60 using 68500-1-Ig (same clone as 68500-1-PBS)

    IP result of anti-PSPH (IP:68500-1-Ig, 4ug; Detection:68500-1-Ig 1:5000) with HL-60 cells lysate 2440 ug. This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using PSPH Monoclonal antibody (68500-1-Ig)

    IHC staining of mouse spleen using 68500-1-Ig (same clone as 68500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68500-1-Ig (PSPH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSPH Monoclonal antibody (68500-1-Ig)

    IF Staining of HepG2 using 68500-1-Ig (same clone as 68500-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (68500-1-Ig, Clone: 1B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

PSPH Monoclonal antibody, PBS Only

PSPH Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 68500-1-PBS
Clone No.1B12G3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PSPase, PSP, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, EC:3.1.3.3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

68500-1-Ig

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68500-1-PBS targets PSPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSPH fusion protein Ag6070 相同性解析による交差性が予測される生物種
Full Name phosphoserine phosphatase
Calculated molecular weight 25 kDa
Observed molecular weight25 kDa
GenBank accession numberBC063614
Gene Symbol PSPH
Gene ID (NCBI) 5723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78330
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).