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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PTBP1 Monoclonal antibody

PTBP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D6D8

Cat no : CL594-67462

Synonyms

PTB, pPTB, HNRPI, HNRNPI, HNRNP I

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated PTBP1 Monoclonal antibody (CL594-67462)

    IF Staining of HepG2 using CL594-67462

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 PTBP1 antibody (CL594-67462, Clone: 3D6D8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated PTBP1 Monoclonal antibody (CL594-67462)

    FC experiment of HeLa using CL594-67462

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PTBP1 (CL594-67462, Clone:3D6D8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67462 targets PTBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTBP1 fusion protein Ag28404 相同性解析による交差性が予測される生物種
Full Name polypyrimidine tract binding protein 1
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC013694
Gene symbol PTBP1
Gene ID (NCBI) 5725
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.

Protocols

Product Specific Protocols
IF protocol for CL594 PTBP1 antibody CL594-67462Download protocol
FC protocol for CL594 PTBP1 antibody CL594-67462Download protocol
Standard Protocols
Click here to view our Standard Protocols