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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTP4A1 Monoclonal antibody

PTP4A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C9E10

Cat no : 67584-1-Ig

Synonyms

PRL1, PTP4A1/PRL1, Protein-tyrosine phosphatase of regenerating liver 1, Protein-tyrosine phosphatase 4a1, PRL-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTP4A1 Monoclonal antibody (67584-1-Ig)

    WB analysis using 67584-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTP4A1 Monoclonal antibody (67584-1-Ig)

    WB analysis using 67584-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PTP4A1 Monoclonal antibody (67584-1-Ig)

    IHC staining of human lung cancer using 67584-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PTP4A1 Monoclonal antibody (67584-1-Ig)

    IHC staining of human lung cancer using 67584-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTP4A1 Monoclonal antibody (67584-1-Ig)

    IF Staining of HeLa using 67584-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTP4A1 antibody (67584-1-Ig, Clone: 2C9E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67584-1-Ig targets PTP4A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTP4A1 fusion protein Ag14436 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase type IVA, member 1
Calculated molecular weight 20 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC023975
Gene symbol PTP4A1
Gene ID (NCBI) 7803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).

Protocols

Product Specific Protocols
WB protocol for PTP4A1 antibody 67584-1-IgDownload protocol
IHC protocol for PTP4A1 antibody 67584-1-IgDownload protocol
IF protocol for PTP4A1 antibody 67584-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols