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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTP4A1 Monoclonal antibody (67584-1-Ig)

    WB analysis using 67584-1-Ig (same clone as 67584-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PTP4A1 Monoclonal antibody (67584-1-Ig)

    WB analysis using 67584-1-Ig (same clone as 67584-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PTP4A1 Monoclonal antibody (67584-1-Ig)

    IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PTP4A1 Monoclonal antibody (67584-1-Ig)

    IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTP4A1 Monoclonal antibody (67584-1-Ig)

    IF Staining of HeLa using 67584-1-Ig (same clone as 67584-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTP4A1 antibody (67584-1-Ig, Clone: 2C9E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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PTP4A1 Monoclonal antibody, PBS Only

PTP4A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67584-1-PBS
Clone No.2C9E10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PRL1, PTP4A1/PRL1, Protein-tyrosine phosphatase of regenerating liver 1, Protein-tyrosine phosphatase 4a1, PRL-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67584-1-PBS targets PTP4A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTP4A1 fusion protein Ag14436 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase type IVA, member 1
Calculated molecular weight 20 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC023975
Gene Symbol PTP4A1
Gene ID (NCBI) 7803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93096
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).