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  • Featured Product
  • KD/KO Validated

SHP2 Recombinant antibody

SHP2 Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5L23

Cat no : 82503-1-RR

Synonyms

PTPN11/SHP2, PTPN11, NS 1, EC:3.1.3.48, CFC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHP2 Recombinant antibody (82503-1-RR)

    WB analysis using 82503-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82503-1-RR (PTPN11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SHP2 Recombinant antibody (82503-1-RR)

    WB analysis of HeLa using 82503-1-RR

    WB result of SHP2 antibody (82503-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHP2 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SHP2 Recombinant antibody (82503-1-RR)

    IP experiment of HEK-293 using 82503-1-RR

    IP result of anti-PTPN11/SHP2 (IP:82503-1-RR, 4ug; Detection:82503-1-RR 1:8000) with HEK-293 cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of rat heart tissue using SHP2 Recombinant antibody (82503-1-RR)

    IHC staining of rat heart using 82503-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using SHP2 Recombinant antibody (82503-1-RR)

    IHC staining of rat heart using 82503-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Affinity and Kinetic Characterization of 82503-1-RR

    Affinity and Kinetic Characterization of 82503-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82503-1-RR against Human PTPN11/SHP2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, A549 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82503-1-RR targets SHP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SHP2 fusion protein Ag13660 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC008692
Gene symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_3086484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN11(protein tyrosine phosphatase, non-receptor type 11), also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, and MGC14433, belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML), and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHP2 antibody 82503-1-RRDownload protocol
IHC protocol for SHP2 antibody 82503-1-RRDownload protocol
IP protocol for SHP2 antibody 82503-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols