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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHP2 Recombinant antibody (82503-1-RR)

    WB analysis using 82503-1-RR (same clone as 82503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82503-1-RR (PTPN11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SHP2 Recombinant antibody (82503-1-RR)

    WB analysis of HeLa using 82503-1-RR (same clone as 82503-1-PBS)

    WB result of SHP2 antibody (82503-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHP2 transfected HeLa cells. This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SHP2 Recombinant antibody (82503-1-RR)

    IP experiment of HEK-293 using 82503-1-RR (same clone as 82503-1-PBS)

    IP result of anti-PTPN11/SHP2 (IP:82503-1-RR, 4ug; Detection:82503-1-RR 1:8000) with HEK-293 cells lysate 1280 ug. This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using SHP2 Recombinant antibody (82503-1-RR)

    IHC staining of rat heart using 82503-1-RR (same clone as 82503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using SHP2 Recombinant antibody (82503-1-RR)

    IHC staining of rat heart using 82503-1-RR (same clone as 82503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82503-1-RR

    Affinity and Kinetic Characterization of 82503-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82503-1-RR against Human PTPN11/SHP2 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

SHP2 Recombinant antibody, PBS Only

SHP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82503-1-PBS
Clone No.5L23

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

PTPN11/SHP2, PTPN11, NS 1, EC:3.1.3.48, CFC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82503-1-PBS targets SHP2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SHP2 fusion protein Ag13660 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06124
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPN11(protein tyrosine phosphatase, non-receptor type 11), also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, and MGC14433, belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML), and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.