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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated PTPN11/SHP2 Monoclonal ant (CL594-66795)

    IF Staining of MCF-7 using CL594-66795

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 PTPN11 antibody (CL594-66795, Clone: 3F8A8 ) at dilution of 1:200.

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CoraLite®594-conjugated SHP2 Monoclonal antibody

SHP2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66795
Clone No.3F8A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66795 targets SHP2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHP2 fusion protein Ag13649 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_2883627
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ06124
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 SHP2 antibody CL594-66795Download protocol
Standard Protocols
Click here to view our Standard Protocols