CoraLite® Plus 750-conjugated PTPN18 Recombinant antibody
PTPN18 Recombinant Antibody for WB, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC (Intra)
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230154G8
Cat no : CL750-83024
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, MCF-7 cells, T-47D cells |
Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL750-83024 targets PTPN18 in WB, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PTPN18 fusion protein Ag34048 相同性解析による交差性が予測される生物種 |
Full Name | protein tyrosine phosphatase, non-receptor type 18 (brain-derived) |
Calculated molecular weight | 460 aa, 50 kDa |
Observed molecular weight | 48 kDa |
GenBank accession number | BC052800 |
Gene symbol | PTPN18 |
Gene ID (NCBI) | 26469 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTPN18 (also called PTP-HSCF or BDP1), a member of the protein tyrosine phosphatase superfamily, has been reported to be a tumor suppressor in breast cancer by dephosphorylating HER2 and suppressing cell proliferation and invasion. In addition to the well-established role of PTPN18 in breast cancer, our previous study found that PTPN18 and CSK cooperate to inhibit the events triggered by SRC kinases. (PMID: 35982039)
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 750 PTPN18 antibody CL750-83024 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |