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  • Featured Product
  • KD/KO Validated

PTPN23 Polyclonal antibody, PBS Only

PTPN23 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10472-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using PTPN23 Polyclonal antibody (10472-1-AP)

    WB analysis of A375 using 10472-1-AP (same clone as 10472-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PTPN23 Polyclonal antibody (10472-1-AP)

    WB analysis of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using PTPN23 Polyclonal antibody (10472-1-AP)

    WB analysis of A375 using 10472-1-AP (same clone as 10472-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PTPN23 Polyclonal antibody (10472-1-AP)

    WB analysis of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10472-1-AP (PTPN23 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PTPN23 Polyclonal antibody (10472-1-AP)

    IP experiment of HeLa using 10472-1-AP (same clone as 10472-1-PBS)

    IP result of anti-PTPN23 (IP:10472-1-AP, 5ug; Detection:10472-1-AP 1:500) with HeLa cells lysate 1000ug. This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN23 Polyclonal antibody (10472-1-AP)

    IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN23 Polyclonal antibody (10472-1-AP)

    IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10472-1-AP (PTPN23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTPN23 Polyclonal antibody (10472-1-AP)

    IHC staining of human colon cancer using 10472-1-AP (same clone as 10472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10472-1-AP (PTPN23 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PTPN23 Polyclonal antibody (10472-1-AP)

    IF Staining of HepG2 using 10472-1-AP (same clone as 10472-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTPN23 antibody (10472-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10472-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10472-1-PBS targets PTPN23 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN23 fusion protein Ag0765 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 23
Calculated molecular weight 179 kDa
Observed molecular weight 179 kDa
GenBank accession numberBC004881
Gene symbol PTPN23
Gene ID (NCBI) 25930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).