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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPRA Polyclonal antibody (13079-1-AP)

    WB analysis using 13079-1-AP (same clone as 13079-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13079-1-AP (PTPRA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using PTPRA Polyclonal antibody (13079-1-AP)

    WB analysis of rat liver using 13079-1-AP (same clone as 13079-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 13079-1-AP (PTPRA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A431 cells using PTPRA Polyclonal antibody (13079-1-AP)

    IP experiment of A431 using 13079-1-AP (same clone as 13079-1-PBS)

    IP result of anti-PTPRA (IP:13079-1-AP, 4ug; Detection:13079-1-AP 1:300) with A431 cells lysate 2400ug. This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PTPRA Polyclonal antibody (13079-1-AP)

    IHC staining of human lung cancer using 13079-1-AP (same clone as 13079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using PTPRA Polyclonal antibody (13079-1-AP)

    IHC staining of human urothelial carcinoma using 13079-1-AP (same clone as 13079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PTPRA Polyclonal antibody (13079-1-AP)

    IHC staining of mouse heart using 13079-1-AP (same clone as 13079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PTPRA Polyclonal antibody (13079-1-AP)

    IHC staining of mouse kidney using 13079-1-AP (same clone as 13079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using PTPRA Polyclonal antibody (13079-1-AP)

    IHC staining of rat heart using 13079-1-AP (same clone as 13079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PTPRA Polyclonal antibody (13079-1-AP)

    IHC staining of rat kidney using 13079-1-AP (same clone as 13079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PTPRA Polyclonal antibody (13079-1-AP)

    IF Staining of A431 using 13079-1-AP (same clone as 13079-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PTPRA antibody (13079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13079-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PTPRA Polyclonal antibody, PBS Only

PTPRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13079-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13079-1-PBS targets PTPRA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRA fusion protein Ag3760 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, A
Calculated molecular weight 793 aa, 91 kDa
Observed molecular weight90-105 kDa, 140 kDa
GenBank accession numberBC027308
Gene Symbol PTPRA
Gene ID (NCBI) 5786
RRIDAB_2269055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18433
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.