CoraLite® Plus 488-conjugated PTPRO Monoclonal antibody
PTPRO Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Pig
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F2B4
Cat no : CL488-67000
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67000 targets PTPRO in IF-P applications and shows reactivity with Human, Pig samples.
| Tested Reactivity | Human, Pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PTPRO fusion protein Ag8284 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, receptor type, O |
| Calculated molecular weight | 138 kDa |
| Observed molecular weight | 160 kDa, 180-220 kDa |
| GenBank accession number | BC035960 |
| Gene symbol | PTPRO |
| Gene ID (NCBI) | 5800 |
| RRID | AB_2919402 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PTPRO antibody CL488-67000 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
