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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPRU Polyclonal antibody (23786-1-AP)

    WB analysis using 23786-1-AP (same clone as 23786-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23786-1-AP (PTPRU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23786-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PTPRU Polyclonal antibody (23786-1-AP)

    IF Staining of U-251 using 23786-1-AP (same clone as 23786-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PTPRU antibody (23786-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23786-1-PBS in a different storage buffer formulation.

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PTPRU Polyclonal antibody, PBS Only

PTPRU Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 23786-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23786-1-PBS targets PTPRU in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRU fusion protein Ag20667 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, U
Calculated molecular weight 1446 aa, 162 kDa
Observed molecular weight170 kDa
GenBank accession numberBC146655
Gene Symbol PTPRU
Gene ID (NCBI) 10076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ92729
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PTPRU (protein tyrosine phosphatase, receptor type, U) is also named as PCP-2,PTP-J, PTP pi, PTP-RO and R-PTP-psi. Protein tyrosine phosphatase plays a crucial role in the regulation of signal transduction during diverse cell functions, including cell activation, proliferation, differentiation, survival, and metabolic homeostasis. PTPases are classified into two groups, membrane-spanning receptor-type (R-PTPases) and cytosolictype. The human PTPRU cDNA clone encodes a polypeptide of 1,439 amino acids and the predicted molecular mass of the PTPRU protein is 162 kDa. It has reported that SCF induced tyrosine phosphorylation of the full-length PTPRU (190 kDa) and the proteolytic forms of PTPRU (100 and 80 kD). A non-tyrosine phosphorylated 73 kDa band was detected in addition to those of 190, 90, and 80 kDa. (PMID: 10397721).