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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTRF Recombinant antibody (82697-1-RR)

    WB analysis using 82697-1-RR (same clone as 82697-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using PTRF Recombinant antibody (82697-1-RR)

    WB analysis of rat lung using 82697-1-RR (same clone as 82697-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PTRF Recombinant antibody (82697-1-RR)

    IHC staining of human stomach using 82697-1-RR (same clone as 82697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82697-1-RR (PTRF antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PTRF Recombinant antibody (82697-1-RR)

    IF Staining of HepG2 using 82697-1-RR (same clone as 82697-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (82697-1-RR, Clone: 3E15 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82697-1-RR

    Affinity and Kinetic Characterization of 82697-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82697-1-RR against Human PTRF were performed. The affinity constant is 0.303 nM.

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PTRF Recombinant antibody, PBS Only

PTRF Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82697-1-PBS
Clone No.3E15

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FKSG13, Cavin-1, Cavin 1, 3E15

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82697-1-PBS targets PTRF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTRF fusion protein Ag13486 相同性解析による交差性が予測される生物種
Full Name polymerase I and transcript release factor
Calculated molecular weight 43 kDa
Observed molecular weight55 kDa
GenBank accession numberBC008849
Gene Symbol PTRF
Gene ID (NCBI) 284119
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NZI2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.