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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PTRF Recombinant antibody

PTRF Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E15

Cat no : 82697-1-RR

Synonyms

FKSG13, Cavin-1, Cavin 1, 3E15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTRF Recombinant antibody (82697-1-RR)

    WB analysis using 82697-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using PTRF Recombinant antibody (82697-1-RR)

    WB analysis of rat lung using 82697-1-RR

    rat lung tissue were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PTRF Recombinant antibody (82697-1-RR)

    IHC staining of human stomach using 82697-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82697-1-RR (PTRF antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PTRF Recombinant antibody (82697-1-RR)

    IF Staining of HepG2 using 82697-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (82697-1-RR, Clone: 3E15 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82697-1-RR

    Affinity and Kinetic Characterization of 82697-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82697-1-RR against Human PTRF were performed. The affinity constant is 0.303 nM.

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Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue, mouse heart tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82697-1-RR targets PTRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTRF fusion protein Ag13486 相同性解析による交差性が予測される生物種
Full Name polymerase I and transcript release factor
Calculated molecular weight 43 kDa
Observed molecular weight55 kDa
GenBank accession numberBC008849
Gene symbol PTRF
Gene ID (NCBI) 284119
RRIDAB_3086511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTRF antibody 82697-1-RRDownload protocol
IHC protocol for PTRF antibody 82697-1-RRDownload protocol
IF protocol for PTRF antibody 82697-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols