CoraLite® Plus 488-conjugated PYCR1 Monoclonal antibody

PYCR1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H2G1

Cat no : CL488-66510

Synonyms

P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2


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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66510 targets PYCR1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PYCR1 fusion protein Ag17919 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase 1
Calculated molecular weight 319 aa, 33.8 kDa
Observed molecular weight 33 kDa, 35 kDa
GenBank accession numberBC022244
Gene symbol PYCR1
Gene ID (NCBI) 5831
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PYCR1 antibody CL488-66510Download protocol
FC protocol for CL Plus 488 PYCR1 antibody CL488-66510Download protocol
Standard Protocols
Click here to view our Standard Protocols