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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 488-conjugated PYCRL Monoclonal ant (CL488-68087)

    FC experiment of HEK-293T using CL488-68087

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PYCRL (CL488-68087, Clone:1G7A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated PYCRL Monoclonal antibody

PYCRL Monoclonal Antibody for FC (Intra)
Cat No. CL488-68087
Clone No.1G7A3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

PYCR3, Pyrroline-5-carboxylate reductase-like protein, Pyrroline-5-carboxylate reductase 3, P5CR 3, P5C reductase 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68087 targets PYCRL in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PYCRL fusion protein Ag31938 相同性解析による交差性が予測される生物種
Full Name pyrroline-5-carboxylate reductase-like
Calculated molecular weight 274 aa, 29 kDa
Observed molecular weight26-29 kDa
GenBank accession numberBC007993
Gene Symbol PYCRL
Gene ID (NCBI) 65263
RRIDAB_2934620
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ53H96
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 PYCRL antibody CL488-68087Download protocol
Standard Protocols
Click here to view our Standard Protocols