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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis of HSC-T6 using 66769-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis using 66769-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis of HeLa using 66769-1-Ig

    WB result of PYGL antibody (66769-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGL transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PYGL Monoclonal antibody (66769-1-Ig)

    IF Staining of U-251 using 66769-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PYGL antibody (66769-1-Ig, Clone: 1G12D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  • KD/KO Validated

PYGL Monoclonal antibody

PYGL Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66769-1-Ig
Clone No.1G12D8

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Glycogen phosphorylase, liver form, EC:2.4.1.1, 1G12D8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHSC-T6 cells, HeLa cells, HepG2 cells, L02 cells, SMMC-7721 cells
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66769-1-Ig targets PYGL in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PYGL fusion protein Ag8550 相同性解析による交差性が予測される生物種
Full Name phosphorylase, glycogen, liver
Calculated molecular weight 846 aa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC009895
Gene Symbol PYGL
Gene ID (NCBI) 5836
RRIDAB_2882115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06737
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen phosphorylase L (PYGL) is one of the gene related to hypoxia metabolism and was found to be up-regulated in head and neck squamous cell carcinomas (HNSCCs) and breast cancers. Glycogen synthases (GSs) and glycogen phosphorylases (GPs) catalyze the key steps of glycogen synthesis and breakdown, while glycogen phosphorylase has three isoforms: PYGM (muscle), PYGL (liver), and PYGB (brain) (PMID: 37063425, 9529348). High PYGL expression plays an independent role in predicting the poor prognosis of glioma patients (PMID: 34177761).

Protocols

Product Specific Protocols
WB protocol for PYGL antibody 66769-1-IgDownload protocol
IF protocol for PYGL antibody 66769-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols