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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis of HSC-T6 using 66769-1-Ig (same clone as 66769-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis using 66769-1-Ig (same clone as 66769-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis of HeLa using 66769-1-Ig (same clone as 66769-1-PBS)

    WB result of PYGL antibody (66769-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGL transfected HeLa cells. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PYGL Monoclonal antibody (66769-1-Ig)

    IF Staining of U-251 using 66769-1-Ig (same clone as 66769-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PYGL antibody (66769-1-Ig, Clone: 1G12D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

PYGL Monoclonal antibody, PBS Only

PYGL Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66769-1-PBS
Clone No.1G12D8

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Glycogen phosphorylase, liver form, EC:2.4.1.1, 1G12D8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66769-1-PBS targets PYGL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PYGL fusion protein Ag8550 相同性解析による交差性が予測される生物種
Full Name phosphorylase, glycogen, liver
Calculated molecular weight 846 aa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC009895
Gene Symbol PYGL
Gene ID (NCBI) 5836
RRIDAB_2882115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06737
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glycogen phosphorylase L (PYGL) is one of the gene related to hypoxia metabolism and was found to be up-regulated in head and neck squamous cell carcinomas (HNSCCs) and breast cancers. Glycogen synthases (GSs) and glycogen phosphorylases (GPs) catalyze the key steps of glycogen synthesis and breakdown, while glycogen phosphorylase has three isoforms: PYGM (muscle), PYGL (liver), and PYGB (brain) (PMID: 37063425, 9529348). High PYGL expression plays an independent role in predicting the poor prognosis of glioma patients (PMID: 34177761).